biotinylated polyclonal anti amphotericin antibody (Vector Laboratories)
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Vector Laboratories
biotinylated polyclonal anti amphotericin antibody
Biotinylated Polyclonal Anti Amphotericin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+polyclonal+anti+amphotericin+antibody/Biotinylated+Universal+Pan-Specific+Antibody+(Horse+Anti-Mouse%2FRabbit%2FGoat+IgG)/pm35540105-154-51-105
Average 93 stars, based on 154 article reviews
Biotinylated Polyclonal Anti Amphotericin Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotinylated+polyclonal+anti+amphotericin+antibody/Biotinylated+Universal+Pan-Specific+Antibody+(Horse+Anti-Mouse%2FRabbit%2FGoat+IgG)/pm35540105-154-51-105
Average 93 stars, based on 154 article reviews
biotinylated polyclonal anti amphotericin antibody - by Bioz Stars,
2026-10
93/100 stars
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Adhesive:Article Title: Generation and use of functionalised hydrogels that can rapidly sample infected surfaces. Article Snippet: 2 ml) with 5 % BSA-PBS-T and place 0.5 cm diameter hydrogel disc (one disc per tube) and incubate overnight at 4 °C Wash the plate and hydrogels with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogel pieces) of the biotinylated monoclonal mouse anti-polymyxin antibody (1:1,0 0 0) with 1% BSA-PBS-T Cover the plate with adhesive plastic and incubate at room temperature for 2 hours Wash the plate with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogels) of ABC reagent prepared according to manufacturers instructions ( http://vectorlabs.com/uk/ vectastain- elite- abc- kit- universal.html?SID=743e1e74ad49a56ec2eb2bc61adcbb86 ) Cover the plate with adhesive plastic and incubate at room temperature for 30 min Wash the plate with washing buffer 4 times Dissolve TMB substrate tablet in 25 ml of 0.05 M phosphate-citrate buffer (pH 5.0) Add 10 μl of 30 % H 2 O 2 into TMB solution Add 100 μl of TMB solution to each well of the plate or 200 μl to the 2.0ml tube ensuring all hydrogel piece is covered with solution After sufficient colour development add 100 μl or 200 μl stop solution (1 M H 2 SO 4 ) (1:1 TMB: stop solution) Measure the absorbance of each well at 450 nm Calculate the concentration of samples from the standard curve Amphotericin ELISA for Hydrogels To a high binding ELISA plate add 100 μl polyclonal anti-amphotericin (1:10 0 0 dilution in 35 mM NaHCO 3 , 15 mM Na 2 CO 3 and 3mM NaN 3 in distilled water) and cover the plate with adhesive plastic Incubate the plate at 4 °C overnight Wash the plate 4 times with 200 μl PBS-Tween 20 (1:200,000 dilution of Tween: PBS) (PBS-T) Add 200 μl of blocking buffer (5 % bovine serum albumin (BSA) in PBS-T) in order to block the non-specific binding sites on the coated well Cover the plate with adhesive plastic and incubate at 4 °C overnight Wash the plate with 200 μl of PBS-T 4 times Prepare standard amphotericin solutions (1:2 dilutions (in mg ml −1 ), e.g., 2.5, 1.25, 0.625, 0.313, 0.156, 0.078, 0.039, 0.019, 0.0097, 0.0048, 0.0024, 0 in 1 % BSA-PBS-T) and add to the plate Cover the plate with adhesive plastic and incubate at 4 °C overnight In a U bottomed Eppendorf tube fill to the top (approx. .. 2 ml) with 5 % BSA-PBS-T and place 0.5 cm diameter hydrogel disc (one disc per tube) and incubate overnight at 4 °C Wash the plate and hydrogels with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogel pieces) of the Concentration Assay:Article Title: Generation and use of functionalised hydrogels that can rapidly sample infected surfaces. Article Snippet: 2 ml) with 5 % BSA-PBS-T and place 0.5 cm diameter hydrogel disc (one disc per tube) and incubate overnight at 4 °C Wash the plate and hydrogels with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogel pieces) of the biotinylated monoclonal mouse anti-polymyxin antibody (1:1,0 0 0) with 1% BSA-PBS-T Cover the plate with adhesive plastic and incubate at room temperature for 2 hours Wash the plate with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogels) of ABC reagent prepared according to manufacturers instructions ( http://vectorlabs.com/uk/ vectastain- elite- abc- kit- universal.html?SID=743e1e74ad49a56ec2eb2bc61adcbb86 ) Cover the plate with adhesive plastic and incubate at room temperature for 30 min Wash the plate with washing buffer 4 times Dissolve TMB substrate tablet in 25 ml of 0.05 M phosphate-citrate buffer (pH 5.0) Add 10 μl of 30 % H 2 O 2 into TMB solution Add 100 μl of TMB solution to each well of the plate or 200 μl to the 2.0ml tube ensuring all hydrogel piece is covered with solution After sufficient colour development add 100 μl or 200 μl stop solution (1 M H 2 SO 4 ) (1:1 TMB: stop solution) Measure the absorbance of each well at 450 nm Calculate the concentration of samples from the standard curve Amphotericin ELISA for Hydrogels To a high binding ELISA plate add 100 μl polyclonal anti-amphotericin (1:10 0 0 dilution in 35 mM NaHCO 3 , 15 mM Na 2 CO 3 and 3mM NaN 3 in distilled water) and cover the plate with adhesive plastic Incubate the plate at 4 °C overnight Wash the plate 4 times with 200 μl PBS-Tween 20 (1:200,000 dilution of Tween: PBS) (PBS-T) Add 200 μl of blocking buffer (5 % bovine serum albumin (BSA) in PBS-T) in order to block the non-specific binding sites on the coated well Cover the plate with adhesive plastic and incubate at 4 °C overnight Wash the plate with 200 μl of PBS-T 4 times Prepare standard amphotericin solutions (1:2 dilutions (in mg ml −1 ), e.g., 2.5, 1.25, 0.625, 0.313, 0.156, 0.078, 0.039, 0.019, 0.0097, 0.0048, 0.0024, 0 in 1 % BSA-PBS-T) and add to the plate Cover the plate with adhesive plastic and incubate at 4 °C overnight In a U bottomed Eppendorf tube fill to the top (approx. .. 2 ml) with 5 % BSA-PBS-T and place 0.5 cm diameter hydrogel disc (one disc per tube) and incubate overnight at 4 °C Wash the plate and hydrogels with washing buffer 4 times Add 100 μl to the wells (standards) or 300 μl to the 2.0ml tube (hydrogel pieces) of the |